Mycoplasma Testing Service (Conventional PCR)
 |
Mycoplasma contamination is most widespread contamination found in cell culture. The small size of Mycoplasma makes them invisible to the naked eye as well as conventional light microscope. Further, presence of Mycoplasma in cultures may not produce visible signs of contamination but can have adverse effects on cell line growth and characteristics. Subsequently, Mycoplasma contamination may influence the experimental results and its reproducibility. In order to achieve the reproducible results from cultured cells, it is advised to test cells for Mycoplasma contamination.
PCR based detection method is widely used for reliable results by reputed organisations and international cell banks. It is one of the most rapid, sensitive and convenient method. The primer set is specific to the highly conserved 16S rRNA coding region in the Mycoplasma genome. The detection spectrum includes most of the species of Mycoplasma usually encountered as contaminants in cell cultures.
Special Note for DNA Sample Preparation:
The DNA sample from cell line to be tested for Mycoplasma contamination should be derived from:
1. Cultures grown without antibiotics for several passages (since it affects the test sensitivity).
2. Continuous cultures (at least one or two passages after thawing) and 80-90 % confluence at the time of DNA extraction.
Mycoplasma Testing Request Form here 
For non-profit organizations: *Rs. 1800/- per sample
For others: *Rs. 3600/- per sample
*Additional charges for GST @18% will be applicable to all.
The charges may kindly be remitted in advance through the following means:
Electronic money transfer to the below-mentioned account:
Account Name: Director, NCCS
Name of the Bank: State Bank of India
Branch Address: NCL Campus Branch, Dr.Homi Bhabha Road, Pashan, Pune-411008
Saving A/c No. : 33794408771
IFSCode : SBIN0003552
Swift Code: SBININBB218
Kindly send the hard copy of the online transaction receipt and your request letter along with DNA sample.
Contact: Dr. Rahul Patil
Email: rahulpatil@nccs.res.in, 020-25708248/8268