Scientist Profile

Vasudevan Seshadri, Ph.D.


Research Areas

Pathogenesis and Cellular Response, Regulatory RNAs and Gene Expression

Education and Experience


  • Ph.D., Microbiology and Cell Biology, 1998, Indian Institute of Science, Bangalore India
  • M.Sc., Biotechnology, 1990, Indian Institute of Technology, Mumbai, India
  • B.Sc. Chemistry, Physics and Mathematics, 1988, Delhi University, India


Research Experience

  • Scientist G, 2022 to present National Centre for Cell Science, Pune, India
  • Scientist F, 2016-2022 National Centre for Cell Science, Pune, India
  • Scientist E, 2010-2016, National Centre for Cell Science, Pune, India
  • Scientist D, 2004 to 2010, National Centre for Cell Science, Pune, India
  • Post doctoral Research Fellow, 1998-2004, Department of Cell Biology, Lerner Research Institute, Cleveland Clinic Foundation, Cleveland, Ohio, USA
  • Research Fellow, 1996-1997, Department of Biochemistry, University of Montreal, Montreal, Quebec, Canada


The complexity of life is far greater than what was expected. This complexity arises through extensive regulation at post-transcription level. My lab studies the mechanism of translation regulation of some key transcripts that are important in diseases like diabetes and malaria. The goal of our lab is to understand gene regulation with specific focus on RNA-protein interactions.


Area of Research

Gene regulation: RNA protein interactions

The broad area of my research lab is to understand how specific RNA-protein interactions regulate gene expression. We have elucidated a role for PDI, PABP and HuD in insulin gene expression. We have also recently reported a novel role for PIP4K2A in regulating gene expression in malaria parasite. We have shown that the RNA binding activity of PIP4K is conserved and have also identified specific sequence motif in the RNA that is essential for this interaction.


Translation regulation of insulin

Insulin plays a central role in glucose metabolism in vertebrates and its biosynthesis is regulated by glucose. Major regulation of insulin occurs at the secretion and the protein synthesis level in β-islet cells. These cells contain a large pool of insulin mRNA (10-15% total mRNA), which is translationally quiescent at hypo-glycaemic (<3 mM glucose) conditions. Recruitment to polysomes and activated translation of this mRNA occur in response to higher glucose levels, leading to about 50-fold increase in insulin biosynthesis within an hour. We have delineated the molecular mechanism underlying the glucose stimulated insulin biosynthesis, and have identified specific RNA binding proteins (PDI, PABP, HuD) that associate with insulin mRNA and regulate its biosynthesis. We have previously shown that prolonged exposure of CHO-Glut4 cells (insulin sensitive cells) to insulin results in them becoming insulin resistant. We have delineated the molecular mechanism of this insulin resistance and our results suggest that insulin exposure results in a altered epigenetic regulation leading impaired PI3K/AKT signalling pathways. We have extended these observations to mice and have developed a model of insulin resistance which could be further used for understanding the molecular basis of diabetes and also screen for drugs that alter insulin sensitivity.


Identifying the RNA binding proteins in Plasmodium falciparum

Plasmodium falciparum is a causative agent for malaria and has a complex life cycle in human and mosquito hosts. During its life cycle, the malarial parasite Plasmodium goes through different asexual stages in human blood, and asexual and sexual stage in mosquito. Expression of stage-specific proteins is important for successful completion of its life cycle and requires tight gene regulation. In case of Plasmodium, due to relative paucity of the transcription factors, it is postulated that post-transcriptional regulation plays an important role in stage-specific gene expression. We have previously shown that human PIP4K2A from RBC is imported into the parasite and is associated with parasite transcripts. We have shown that the RNA binding activity of PIP4K is conserved as PIP4k from Drosophila, C. elegans and Zebra fish show RNA binding activity. The RNA Binding and kinase function of PIP4K2A may be independent as kinase mutants still show RNA binding activity. Our results suggest that the stem loop structure and the multiple UUGU motif present in the RNA is necessary for the RNA-protein interactions.  to be recognized by PIP4K2A. In order to understand the other targets of PIP4K2A we have performed RNA-Clip experiments followed by sequencing using mammalian cells as we believe that the RNA binding activity of PIP4K2A plays and important role in mammalian gene regulation.





Jatin Behari, Pranita Borkar, Arya Vindu, Vishal Dandewad, Sindhuri Upadrasta, Dhanshekaran Shanmugam, Vasudevan Seshadri (2021) Conserved RNA binding activity of Phoshatidyl inositol 5-phosphate 4-kinase (PIP4K2) Frontiers in Molecular Biosciences

Ridim D Mote 1, V Shinde Laxmikant, Surya Bansi Singh, Mahak Tiwari, Hemant Singh, Juhi Srivastava, Vidisha Tripathi, Vasudevan Seshadri, Amitabha Majumdar, Deepa Subramanyam (2021) A cost-effective and efficient approach for generating and assembling reagents for conducting real-time PCR. J Biosci. 2021;46(4):109.  doi: 10.1007/s12038-021-00231-w

Dushyant Kumar Srivastava, Sarika Gunjan, Chandrima Das, Vasudevan Seshadri and  Siddhartha Roy (2021) Structural insights into histone chaperone Asf1 and its characterization from Plasmodium falciparum Biochem J BCJ20200891



Bhargab Kalita.; Shehnaz Bano, Vijayakumar M Vavachan, Khushman Taunk, Vasudevan Seshadri and Srikanth Rapole (2020) Application of mass spectrometry based proteomics to understand diabetes: A special focus on interactomics. BBA - Proteins and Proteomics Oct;1868(10):140469. doi: 10.1016/j.bbapap.2020.140469

Indumathi Patta, Ayush Madhok, Satyajeet Khare, Kamalvishnu P Gottimukkala, Anjali Verma, Shilpi Giri, Vishal Dandewad, Vasudevan Seshadri, Girdhari Lal, Jyoti Misra-Sen, Sanjeev Galande (2020) Dynamic regulation of chromatin organizer SATB1 via TCR-induced alternative promoter switch during T-cell development Nucleic Acids Research, 48 (11) 5873–5890, 19 June 2020,

Rucha D. Sarwade, Abdul Khalique, Shardul D. Kulkarni, Poonam R. Pandey, Naina Gaikwad and Vasudevan Seshadri (2020) Translation of insulin granule proteins are regulated by PDI and PABP Biochem Biophys Res Commun. 526 (3), 618-625, 4 June 2020



Dandewad V, Vindu A, Joseph J and Seshadri V. (2019) Import of human miRNA-RISC complex into Plasmodium falciparum and regulation of the parasite gene expression. Journal of Bioscience 44:50, DOI: 10.1007/s12038-019-9870-x



Kadam S, Kanitkar M, Dixit K, Deshpande R, Seshadri V, Kale V. (2018) Curcumin reverses diabetes-induced endothelial progenitor cell dysfunction by enhancing MnSOD expression and activity in vitro and in vivo. J Tissue Eng Regen Med. Jul;12(7):1594-1607. doi: 10.1002/term.2684. Epub 2018 May 18.

Suniti Vaishya, Rucha D. Sarwadea and Vasudevan Seshadri (2018)  miRNA, proteins and metabolites as novel biomarkers for pre-diabetes, diabetes and related complications Frontiers in Endocrinology

Pandey PR, Sarwade RD, Khalique A, Seshadri V. (2018) Interaction of HuDA and PABP at 5'UTR of mouse insulin2 regulates insulin biosynthesis. PLoS One;13(3):e0194482. doi: 10.1371/journal.pone.0194482. ECollection 2018.

Vindu A, Dandewad V, Seshadri V. (2018) Identification of human Phosphatidyl Inositol 5-Phosphate 4-Kinase as an RNA binding protein that is imported into Plasmodium falciparum. Biochem Biophys Res Commun.;498(3):529-536. doi: 10.1016/j.bbrc.2018.03.014. Epub 2018 Mar 6.



Manne RK, Agrawal Y, Bargale A, Patel A, Paul D, Gupta NA, Rapole S, Seshadri V, Subramanyam D, Shetty P, Santra MK (2017). A MicroRNA/Ubiquitin Ligase Feedback Loop Regulates Slug-Mediated Invasion in Breast Cancer. Neoplasia. 19(6):483-495. doi: 10.1016/j.neo.2017.02.013.



Sahoo MR, Gaikwad S, Khuperkar D, Ashok M, Helen M, Yadav SK, Singh A, Magre I, Deshmukh P, Dhanvijay S, Sahoo PK, Ramtirtha Y, Madhusudhan MS, Gayathri P, Seshadri V, Joseph J (2016) Nup358 binds to AGO proteins through its SUMO-interacting motifs and promotes the association of target mRNA with miRISC. EMBO Rep. pii: e201642386. doi: 10.15252/embr.201642386.

Abdul Khalique, Rucha D. Sarwade, Poonam R. Pandey, M.V. Vijayakumar, Manoj K. Bhat and  Vasudevan Seshadri (2016) Prolonged exposure to insulin with insufficient glucose leads to impaired Glut4 translocation. Biochemical and Biophysical Research Communications 474 (2016) 64-70


Before 2016

Poonam Pandey and Vasudevan Seshadri (2014) Role of miR-200a in regulating the insulin signalling in the hypothalamus. Non-coding RNAs in Endocrinology, 1:, 44-46
Amaresh C. Panda, Itishri Sahu, Shardul D. Kulkarni, Jennifer L. Martindale, Kotb Abdelmohsen, Arya Vindu, Jomon Joseph, Myriam Gorospe and Vasudevan Seshadri (2014) miR-196b-mediated translation regulation of mouse Insulin2 via the 5’UTR. PLoS One, 9 (7) e101084, 1-11
Venkatesan V, Madhira SL, Malakapalli VM, Chalasani M, Shaik SN, Seshadri V, Kodavalla V, Bhonde RR, Nappanveettil G., (2013) Obesity, insulin resistance, and metabolic syndrome: a study in WNIN/Ob rats from a pancreatic perspective. Biomed Res Int. 2013; 2013:617569. doi: 10.1155/2013/617569. PMID: 24455710
Mitnala Sasikala, Guduru Venkat Rao, Venkateshan Vijayalakshmi, Rebala Pradeep, Suresh Pothani, Pondugala Pavan Kumar, Radhika Gaddipati, Ganneru Sirisha, Ramji Cheemalakonda, Manu Tandan, Chivukula Subramanyam, Seshadri Vasudevan, and D. Nageshwar Reddy (2013) Long-Term Functions of Encapsulated Islets Grafted in Nonhuman Primates Without Immunosuppression Transplantation 96(7):624-632
Sangeeta Chatterjee, Amaresh C. Panda, Sunil K. Berwal, Sreejith, R.K, Charu Ritvika, Vasudevan Seshadri and Jayanta K. Pal (2013) Vimentin is a component of complex that binds to the 5′-UTR of human heme-regulated eIF2α kinase mRNA and regulates its translation. FEBS Lett. 587(5):474-80
Pabitra K. Sahoo, Prayag Murawala, Pravin T. Sawale, Manas R. Sahoo, Mukesh M. Tripathi, Swati R. Gaikwad, Vasudevan Seshadri and Jomon Joseph (2012) Wnt signalling antagonizes stress granule assembly through a Dishevelled-dependent mechanism. Biology Open 1(2):109-19.
Shardul D. Kulkarni*, Bhavana Muralidharan*, Amaresh C. Panda, Baskar Bakthavachalu, Arya Vindu and Vasudevan Seshadri (2011) Glucose-stimulated Translation Regulation of Insulin by the 5′ UTR-binding Proteins. J. Biol. Chem. 286: 14146-14156
Amaresh C. Panda, Shardul D. Kulkarni, Bhavana Muralidharan, Baskar Bakthavachalu and Vasudevan Seshadri (2010) Novel splice variant of mouse insulin2 mRNA: Implications for insulin expression. FEBS Lett. 584: 1169–1173
Baskar B, Sarmishtha Kalanke, Sanjeev Galande, Ramanamurthy B., Kalidas Kohale and Vasudevan Seshadri (2010) Dense Cataract and Microphthalmia (dcm) in BALB/c Mice is caused by mutations in the GJA8 locus. J. Genetics 89: (2) 147-154
Muralidharan B, Bakthavachalu B, Pathak A, Vasudevan Seshadri. (2007) A minimal element in 5'UTR of insulin mRNA mediates its translational regulation by glucose. FEBS Lett.; 581(21): 4103-8.
Sampath P, Mazumder B, Vasudevan Seshadri , Gerber CA, Chavatte L, Kinter M, Ting SM, Dignam JD, Kim S, Driscoll DM, Fox PL. (2004) Noncanonical Function of Glutamyl-Prolyl-tRNA Synthetase; Gene-Specific Silencing of Translation. Cell 119: (2) 195-208.
Barsanjit Mazumder, Prabha Sampath, Vasudevan Seshadri, Ratan K. Maitra, Paul E DiCorleto and Paul L. Fox, (2003) Regulated Release of L13a from 60S Ribosomal subunit: Mechanism of transcript-specific translational control. Cell115: (2) 187-198
Joydeep Sarkar, Vasudevan Seshadri, Nicholas A. Tripoulas, Michael E. Ketterer, and Paul L. Fox, (2003) Role of ceruloplasmin in macrophage iron efflux during hypoxia. J. Biol. Chem. 278: (45) 44018-24
Prabha Sampath, Barsanjit Mazumder, Vasudevan Seshadri and Paul L. Fox, (2003) Transcript-selective translational silencing by interferon gamma is directed by a novel structural element in the ceruloplasmin mRNA 3’-UTRMol. Cell. Biol. 23: 1509-19
Barsanjit Mazumdar, Vasudevan Seshadri, Paul L. Fox, (2003) Translation control by the 3’UTR: The ends specify the means. Trends in Biochem. Sci.28: 91-98
Vasudevan Seshadri, Paul L. Fox, and Chinmay K. Mukhopadhyay, (2002) Dual role of insulin in transcriptional regulation of the acute phase reactant ceruloplasmin. J. Biol. Chem. 277: 27903-11
Mazumder B, Vasudevan Seshadri, Imataka H, Sonenberg N, Paul L. Fox. (2001) Translational Silencing of Ceruloplasmin Requires the Essential Elements of mRNA Circularization: Poly(A) Tail, Poly(A)-Binding Protein, and Eukaryotic Translation Initiation Factor 4G. Mol. Cell. Biol. 19: 6440-49.
Zouhair K. Attieh, Chinmay K. Mukhopadhyay, Vasudevan Seshadri, Nicholas A. Tripoulas and Paul L. Fox (1999) Ceruloplasmin Ferroxidase activity stimulates cellular Iron uptake by trivalent cation-specific transport mechanism. J. Biol. Chem274: 1116-23.
Vasudevan Seshadri, Vaijayanti C. Vaidya and Usha Vijayraghavan (1996) Genetic studies of the PRP17 gene of Saccharomyces cerevisiae: A domain essential for function maps to non conserved region of the protein. Genetics 143: 45-55
Vaijayanti C. Vaidya, Vasudevan Seshadri and Usha Vijayraghavan (1996) An extragenic suppresser of prp24-1 defines genetic interaction between PRP24 and PRP21 gene products of Saccharomyces cerevisiaeMol. Gen. Genet. 250: 267-76
Vasudevan Seshadri and Usha Vijayraghavan (1995) A method for rapid mapping of mutations by plasmid rescue strategy in Saccharomyces cerevisiaeJ. Genetics 74: (3) 127-38
Rao C. P., Sarkar P. S., Kaiwar S. and Vasudevan Seshadri (1990) Chromate reductase activity: Characterization of Cr(VI) to Cr(III) conversion. Proc. Indian Acad. Sci. (Chem. Sci.) 102: 219-30

Current/Past Lab Members

Graduate Students

Naina Gaikwad
Shyam More
Gaurav Agarwal
Sourav Halder


Lab Alumni

Bhavana Muralidharan (2010), Scientist, Instem, Bengaluru, India
Baskar Baktavachalu (2010), Assistant Professor, IIT Mandi, India
Amaresh K. Panda, (2011), Scientist, ILS, Bhubhaneshwar, India
Shardul Kulkarni, (2012), Assistant Research Professor, Pennsylvania, USA
Arya Vindu, (2015), Postdoctoral Fellow, NIH, USA
Abdul Khalique, (2016), Postdoctoral Fellow, NIH, USA
Poonam Pandey, (2016), Associate Scientist, Columbia University, USA
Vishal Dandewad (2018), Postdoctoral Fellow, NIH, USA
Rucha Sarwade, (2019), Postdoctoral Fellow, Monash University, Australia
Shehnaz Bano, (2021), Postdoctoral Fellow, University of Pittsburgh, USA
Jatin Behari, (2021), Postdoctoral Fellow, NUS, Singapore
Pranita Borkar (2022), Postdoctoral Fellow, Texas A&M University


Last updated On : 18 October 2022 10:13

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